The SOFTEC Adenovirus RT-qPCR Detection Kit is used for qualitative detection of Adenovirus with DNA isolated from human nasopharyngeal swab, oropharyngeal swab, and whole blood samples. The sensitivity and accuracy optimizations of the kit were performed by real-time one step PCR experiments using hydrolysis probes of adenovirus DNA targets. The working principle of the SOFTEC Adenovirus RT-qPCR Detection Kit is based on the detection of fluorescent signals in the presence of target nucleotide sequences of DNA. PCR for this process takes place in 3 steps. First step: meltdown. In this step, the DNA becomes highly denatured, and its strands separate. Step two: bonding. In this step, the primers are attached to their target sequences on the DNA. Step three: elongation. In this step, the primers that hold on to the target sequences are extended. These described steps are for a PCR cycle. The cycle repeats until an efficient and sufficient concentration is achieved. Adenovirus specific primers are used for the amplification of target sequences and target-specific probes are used for the detection of amplified DNA. The probes are oligonucleotides with a fluorescent probe attached to the 5′ end and an extinguisher attached to the 3′ end. The adenovirus-specific probe is labeled with fluorophore FAM. The probe specific to internal control is labeled with fluorophore HEX. During elongation, DNA polymerase separates the reporter and the extinguisher. With this separation, the fluorescence signal is emitted and measured in real time. SOFTEC Adenovirus RT-qPCR Detection Kit reaches the user in a single tube that contains all the necessary components to perform RT-qPCR.