The SOFTEC SARS-CoV-2 Detection RT-qPCR kit is intended for in vitro diagnosis of SARS-CoV-2 nucleic acids from nasopharyngeal aspirate/lavage, bronchoalveolar lavage, nasopharyngeal swab, and oropharyngeal swab qualitatively.
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The SOFTEC SARS-CoV-2 Detection RT-qPCR kit is intended for in vitro diagnosis of SARS-CoV-2 nucleic acids from nasopharyngeal aspirate/lavage, bronchoalveolar lavage, nasopharyngeal swab, and oropharyngeal swab qualitatively.
| SOFTEC COVID-19 RT-qPCR Kit is used for the qualitative detection of the SARS-CoV-2 (2019- nCoV) virus by RNA obtained from human nasopharyngeal swab, oropharyngeal swab, nasal swab, and oral/saliva swab samples. The kit is optimized for sensitive and accurate quantification of Sars-Cov-2 RNA targets by real-time one- step RT-qPCR using hydrolysis probes. SOFTEC COVID-19 RT-qPCR Kit principle is based upon the transcription of RNA to complementary DNA (cDNA) using reverse-transcriptase (RT). This cDNA is used as template for real time RT-QPCR. The cDNA target sequences are amplified utilizing DNA polymerase by PCR process. PCR has three steps: the first step is melting, DNA is denatured at high temperature yielding to single stranded; the second step is annealing, target specific primers anneal to the target sequence in cDNA; the last and third step is elongation that primers attached to target sequences are extended by DNA polymerase. This is for one PCR cycle and until obtaining efficient DNA concentration the cycle repeats. SARS-CoV-2 specific area-based primers for amplification of target sequences and target specific probes are used for the detection of amplified DNA. Probes are oligonucleotides that have fluorescent probe attached to the 5’end, and a quencher to 3’end. SARS-CoV-2 specific probes are labelled with fluorophore FAM. Internal control specific probe is labeled with the fluorophore VIC/HEX. Throughout the elongation, DNA polymerase cleaves the reporter and quencher by this separation the fluorescence is emitted and measured. SOFTEC COVID-19 RT-qPCR Kit comes in 4 different tubes and contains all the components necessary to perform RT-qPCR in a single tube on qPCR cyclers. |
